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Home >> Antibodies >> APEX2 Antibody [Discontinued, view alternatives]

APEX2 Antibody [Discontinued, view alternatives] (R32794)

  Catalog No Formulation Size Price (USD)  
Image R32794 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of rat kidney lysate with APEX2 antibody at 0.5ug/ml. Predicted molecular weight ~57 kDa.
Availability Discontinued
Species Reactivity Rat
Predicted Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q9UBZ4
Applications Western Blot : 0.5-1ug/ml
Limitations This APEX2 antibody is available for research use only.
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Description

APEX2, also called apurinic/apyrimidinic endonuclease like-2, is a member of the apurinic/apyrimidinic (AP) family of endonucleases that initiate the repair of AP sites formed by spontaneous hydrolysis of the N-glycosylic bond, mutagen-induced base release, or damaged-base excision by a DNA repair glycosylase. RT-PCR detected APEX2 expression in HeLa cells, Jurkat cells, and human kidney, brain and fetal brain tissue. The APEX2 gene is mapped to chromosome Xp11.21. APEX2 participates in both nuclear and mitochondrial base excision repair (BER) and it can play a role in processing 3-prime-damaged termini or 3-prime-mismatched nucleotides. Additionally, APEX2 displayed weaker AP site-specific and 3-prime nuclease activities compared to APEX1.

Application Notes

Optimal dilution of the APEX2 antibody should be determined by the researcher.

Immunogen

Amino acids L102-A210 from the human protein were used as the immunogen for the APEX2 antibody.

Storage

After reconstitution, the APEX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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