APEX1 Antibody / AP Endonuclease Antibody (R30567)

APEX1 Antibody Staining of Human Lung Cancer Tissue IF. Immunofluorescence staining of FFPE human lung cancer tissue with APEX1 Antibody shows strong red signal throughout the tumor, with prominent nuclear staining and additional cytoplasmic immunoreactivity. HIER was performed by steaming sections in pH 8 EDTA buffer for 20 min before antibody testing.

APEX1 Antibody Staining of Rat Liver Tissue IF. Immunofluorescence staining of FFPE rat liver tissue with APEX1 Antibody shows red signal concentrated predominantly in hepatocyte nuclei, with comparatively limited surrounding cytoplasmic fluorescence. HIER was performed by steaming sections in pH 8 EDTA buffer for 20 min before antibody testing.

APEX1 Antibody Staining of U-2 OS Osteosarcoma Cells IF. Immunofluorescence staining of FFPE human U-2 OS osteosarcoma cells with APEX1 Antibody shows strong green signal predominantly localized to the nuclei, consistent with the major nuclear localization of AP endonuclease 1. HIER was performed by steaming in pH 6 citrate buffer for 20 min before antibody testing.

APEX1 Antibody Staining of Human Colon Adenocarcinoma IHC. Immunohistochemistry testing of FFPE human colon adenocarcinoma with APEX1 Antibody shows brown immunoreactivity within the malignant glandular epithelium, including prominent nuclear and cytoplasmic staining. HIER was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before antibody testing.

AP Endonuclease Antibody Staining of Human Spleen IHC. Immunohistochemistry testing of FFPE human spleen with AP Endonuclease Antibody shows widespread brown immunoreactivity among splenic cells, with staining visible against the blue hematoxylin-counterstained background. HIER was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before antibody testing.

APEX1 Antibody Staining of Human Prostate Cancer IHC. Immunohistochemistry testing of FFPE human prostate cancer with APEX1 Antibody shows prominent brown staining of the malignant glandular epithelium, with nuclear and cytoplasmic immunoreactivity and comparatively less staining in surrounding stromal areas. HIER was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before antibody testing.

APEX1 Antibody Staining of Mouse Liver IHC. Immunohistochemistry testing of FFPE mouse liver with APEX1 Antibody shows brown staining throughout the hepatic parenchyma, including readily visible nuclear immunoreactivity in hepatocytes. HIER was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before antibody testing.

AP Endonuclease Antibody Staining of Rat Liver IHC. Immunohistochemistry testing of FFPE rat liver with AP Endonuclease Antibody shows widespread brown staining throughout the hepatic parenchyma, with prominent nuclear immunoreactivity in hepatocytes. HIER was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before antibody testing.

APEX1 Antibody Detection in Human, Rat and Mouse Cell Lines WB. Western blot testing of human K562 (lane 1), HepG2 (lane 2), Raji (lane 3) and RT4 (lane 4), rat C6 (lane 5) and PC-12 (lane 6), and mouse HEPA1-6 (lane 7), RAW264.7 (lane 8) and ANA-1 (lane 9) cell lysates with APEX1 Antibody shows a discrete band at approximately 35-38 kDa across all nine samples, consistent with the predicted molecular weight of approximately 38 kDa for AP endonuclease 1. Signal intensity varies among the samples, with particularly strong detection in several human cell lines.