Ref-1 Antibody / Redox Factor 1 Antibody (R31549)

Ref-1 Antibody Detection in Human, Rat and Mouse Samples WB. Western blot testing of rat NRK (lane 1), human HeLa (lane 2), rat PC-12 (lane 3), rat RH35 (lane 4), mouse HEPA (lane 5), human MCF7 (lane 6), human A549 (lane 7), human placenta (lane 8) and human A431 (lane 9) lysates with Ref-1 Antibody shows a strong, discrete band at approximately 38 kDa across all nine samples, consistent with the expected molecular weight of APE1/APEX1.

Ref-1 Antibody Detection of Recombinant Human APE1 WB. Western blot testing of 0.5 ng recombinant human APE1 protein with Ref-1 Antibody demonstrates recognition of the recombinant target, providing additional evidence of APE1/APEX1 binding.

Ref-1 Antibody Staining of Human Lung Cancer IHC. Immunohistochemistry testing of FFPE human lung cancer tissue with Ref-1 Antibody shows prominent brown nuclear staining in tumor cells, with additional cytoplasmic immunoreactivity. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min before antibody testing.

Redox Factor 1 Antibody Staining of Mouse Brain IHC. Immunohistochemistry testing of FFPE mouse brain tissue with Redox Factor 1 Antibody shows distinct brown nuclear staining in numerous cells, with comparatively limited background staining. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min before antibody testing.

Ref-1 Antibody Staining of Rat Brain IHC. Immunohistochemistry testing of FFPE rat brain tissue with Ref-1 Antibody shows discrete brown nuclear immunoreactivity in a subset of cells against a lightly counterstained tissue background. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min before antibody testing.

Ref-1 Antibody Staining of U-2 OS Osteosarcoma Cells IF. Immunofluorescence staining of FFPE human U-2 OS osteosarcoma cells with Ref-1 Antibody at 2 ug/ml shows strong green APE1/APEX1 signal concentrated predominantly in the nuclei, corresponding closely with blue DAPI nuclear staining. HIER was performed by steaming in pH 6 citrate buffer for 20 min before antibody testing.

Ref-1 Antibody Detection in U937 Histiocytic Lymphoma Cells FACS. Flow cytometry testing of human U937 histiocytic lymphoma cells with Ref-1 Antibody at 1 ug/million cells following goat serum blocking. The antibody-stained population (blue) shows a pronounced rightward fluorescence shift relative to the isotype control (green) and cells-alone control (red), demonstrating detection of APE1/Ref-1.

Redox Factor 1 Antibody Staining of Human Colon Cancer Tissue IF. Immunofluorescence staining of FFPE human colon cancer tissue with Redox Factor 1 Antibody shows strong green immunoreactivity throughout the malignant glandular epithelium, with heterogeneous staining intensity among tumor cells. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min before antibody testing.