APC Antibody rEMM43 | Adenomatous Polyposis Coli

	Catalog No	Formulation	Size	Price (USD)
	V5912-100UG	0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide	100 ug	559
Formulation
	V5912-20UG	0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide	20 ug	259
Formulation
	V5912SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

APC Antibody rEMM43 Staining of Human Testis IHC. Immunohistochemistry testing of FFPE human testis with APC Antibody clone rEMM43 shows prominent brown cytoplasmic staining in germ cells within the seminiferous tubules, with comparatively limited staining in surrounding stromal tissue. Nuclei are counterstained blue. The inset PBS-only negative control shows minimal background signal, supporting the specificity of the observed Adenomatous Polyposis Coli Antibody staining.

APC Antibody rEMM43 Purity Analysis by SDS-PAGE. SDS-PAGE analysis of purified recombinant APC Antibody clone rEMM43 under reduced (R) and non-reduced (NR) conditions with 2 ug antibody loaded per lane. Under reducing conditions, the antibody resolves into bands corresponding to the immunoglobulin heavy and light chains at approximately 50-55 kDa and 25 kDa, respectively. Under non-reducing conditions, the intact antibody migrates at approximately 150-160 kDa, supporting the expected molecular integrity and purity of the recombinant monoclonal antibody preparation.

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APC Antibody rEMM43 Staining of Human Testis IHC. Immunohistochemistry testing of FFPE human testis with APC Antibody clone rEMM43 shows prominent brown cytoplasmic staining in germ cells within the seminiferous tubules, with comparatively limited staining in surrounding stromal tissue. Nuclei are counterstained blue. The inset PBS-only negative control shows minimal background signal, supporting the specificity of the observed Adenomatous Polyposis Coli Antibody staining.

APC Antibody rEMM43 Purity Analysis by SDS-PAGE. SDS-PAGE analysis of purified recombinant APC Antibody clone rEMM43 under reduced (R) and non-reduced (NR) conditions with 2 ug antibody loaded per lane. Under reducing conditions, the antibody resolves into bands corresponding to the immunoglobulin heavy and light chains at approximately 50-55 kDa and 25 kDa, respectively. Under non-reducing conditions, the intact antibody migrates at approximately 150-160 kDa, supporting the expected molecular integrity and purity of the recombinant monoclonal antibody preparation.

Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Recombinant Mouse Monoclonal
Isotype	Mouse IgG2b, kappa
Clone Name	rEMM43
UniProt	P25054
Localization	Adherens junction, Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations	This APC Antibody rEMM43 / Adenomatous Polyposis Coli Antibody is available for research use only.
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Description

APC Antibody rEMM43 / Adenomatous Polyposis Coli Antibody recognizes APC, a large tumor suppressor protein encoded by the APC gene. Adenomatous polyposis coli is a central negative regulator of canonical Wnt signaling and has important functions in epithelial homeostasis, cell polarity, migration and cytoskeletal organization. Loss of normal APC function is strongly associated with colorectal tumorigenesis and inherited familial adenomatous polyposis.

APC controls Wnt signaling largely through regulation of beta-catenin. The protein participates in a destruction complex with AXIN, GSK3 and other regulatory proteins that promotes phosphorylation and subsequent degradation of beta-catenin. When APC function is lost, beta-catenin can accumulate and drive transcription of Wnt-responsive genes involved in cellular proliferation and differentiation. APC mutations therefore provide a direct molecular connection between disruption of Wnt pathway control and the development of colorectal neoplasia.

Beyond beta-catenin regulation, APC interacts with components of the microtubule and actin cytoskeleton and contributes to cell migration, adhesion and maintenance of cellular architecture. The protein can associate with growing microtubule ends and is found at several cellular locations, including the cytoplasm, cell membrane, adherens junctions and cytoskeleton. These functions demonstrate that APC acts as both a signaling regulator and an organizer of cellular structure.

Alterations of the APC gene are particularly important in colorectal cancer. Germline pathogenic variants cause familial adenomatous polyposis, while somatic APC alterations occur frequently during sporadic colorectal tumor development. Researchers studying APC expression, tumor suppressor biology and related cancer-associated pathways can explore additional targets on our Cancer Marker Antibodies page.

Clone rEMM43 is a recombinant mouse monoclonal antibody generated against a C-terminal region of human Adenomatous polyposis coli. Recombinant production provides a defined antibody sequence and supports consistency between production lots. NSJ Bioreagents offers this APC Antibody for research into APC protein expression and the molecular biology of this important tumor suppressor.

Researchers studying APC expression, tumor suppressor function and colorectal cancer biology can explore related targets on our Cancer Marker Antibodies page.

Application Notes

1. Optimal dilution of the APC Antibody rEMM43 / Adenomatous Polyposis Coli Antibody should be determined by the researcher.

2. This APC/Adenomatous polyposis coli antibody is recombinantly produced by expression in CHO cells.

Immunogen

Prokaryotic recombinant protein corresponding to a 251 amino acid region of the C-terminus of the human adenomatous polyposis coli protein was used as the immunogen for the APC/Adenomatous polyposis coli antibody.