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Home >> Antibodies >> APAF1 Antibody / Apoptotic protease-activating factor 1

APAF1 Antibody / Apoptotic protease-activating factor 1 [clone BHF-1] (RQ4704)

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Image RQ4704 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with APAF1 antibody. Predicted molecular weight ~140 kDa.
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Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with APAF1 antibody. Predicted molecular weight ~140 kDa.
IHC staining of FFPE mouse kidney tissue with APAF1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human, Mouse
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name BHF-1
Purity Affinity purified
UniProt O14727
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This APAF1 antibody is available for research use only.
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  • Applications : WB, FACS, Direct ELISA
    Reactivity : Human

Description

Apoptotic Peptidase Activating Factor 1 is a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis.

Application Notes

Optimal dilution of the APAF1 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Apoptotic Peptidase Activating Factor 1 was used as the immunogen for the APAF1 antibody.

Storage

Store the APAF1 antibody at -20oC.

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