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Home >> Antibodies >> APAF1 Antibody [Discontinued, view alternatives]

APAF1 Antibody [Discontinued, view alternatives] (R30355)

  Catalog No Formulation Size Price (USD)  
Image R30355 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 299
Western blot testing of APAF1 antibody and Lane 1: rat brain; 2: COLO320; 3: MCF-7; Predicted size: 140KD; Observed size: 140KD
Availability Discontinued
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt O14727
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This APAF1 antibody is available for research use only.
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Description

Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23. It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis. This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain(CARD), and an ATPase domain(NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9. Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death. Additionally, APAF1 has been shown to interact with NLRP1, Caspase-9, APIP, BCL2-like 1 and HSPA4.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the APAF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of human APAF1 (MDAKARNCLLQHREALEKD) was used as the immunogen for this APAF1 antibody (100% homologous in human and mouse).

Storage

After reconstitution, the APAF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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