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Home >> Antibodies >> AP1M1 Antibody

AP1M1 Antibody (F54275)

  Catalog No Formulation Size Price (USD)  
Image F54275-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 369
Image
F54275-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot testing of 1) human PC-3, 2) human U-251 MG, 3) mouse NIH 3T3 and 4) mouse heart lysate with AP1M1 antibody. Predicted molecular weight ~49 kDa.
Western blot testing of human brain lysate with AP1M1 antibody. Predicted molecular weight ~49 kDa.
IHC testing of FFPE human brain tissue with AP1M1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human A375 cells with AP1M1 antibody (green) and DAPI nuclear stain (blue).
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9BXS5
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Immunofluorescence : 1:25
Immunohistochemistry (FFPE) : 1:25
Limitations This AP1M1 antibody is available for research use only.
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Description

The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].

Application Notes

The stated application concentrations are suggested starting points. Titration of the AP1M1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 199-227 from the human protein were used as the immunogen for the AP1M1 antibody.

Storage

Aliquot the AP1M1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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