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Home >> Antibodies >> ANXA1 Antibody / Annexin A1

ANXA1 Antibody / Annexin A1 [clone rANXA1/9145] (V5843)

  Catalog No Formulation Size Price (USD)  
Image V5843-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V5843-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V5843SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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ANXA1 Antibody Human, Mouse, Rat, Hamster, Guinea Pig and Rabbit Lung WB. Western blot analysis of human, mouse, rat, hamster, guinea pig and rabbit lung tissue lysates using ANXA1 Antibody (clone rANXA1/9145). Annexin A1 is detected at approximately 36-42 kDa across the species tested, with a doublet observed in several samples. The additional band may reflect post-translational modification or proteolytic processing of Annexin A1.
ANXA1 Antibody Human A549 Lung Cancer Cell and Tissue WB. Western blot analysis of human intestine, human lung and A549 lung cancer cell lysates using ANXA1 Antibody (clone rANXA1/9145). Annexin A1 is detected at approximately 37-42 kDa across the samples tested, supporting detection of ANXA1 in human tissues and A549 lung cancer cells.
ANXA1 Antibody Human A549 Lung Cancer Cell WB. Western blot analysis of human A549 lung cancer cell lysate using ANXA1 Antibody (clone rANXA1/9145). A strong band is detected at approximately 36 kDa, consistent with the predicted molecular weight of Annexin A1. This ANXA1 Antibody supports detection of Annexin A1 in human lung cancer cells.
ANXA1 Antibody Human Tonsil Immune Cell IHC. Immunohistochemistry staining of FFPE human tonsil tissue with ANXA1 Antibody (clone rANXA1/9145). Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before staining. Strong ANXA1 staining is observed in immune cell populations within the tonsil tissue. The inset shows the corresponding negative control.
SDS-PAGE analysis of purified, BSA-free ANXA1 antibody (clone rANXA1/9145) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rANXA1/9145
Purity Protein A/G affinity
UniProt P04083
Localization Nucleus, Cytoplasm
Applications Western Blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This ANXA1 antibody is available for research use only.
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Description

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Researchers studying Annexin A1 expression and function can explore our Protein Microarray Validated Annexin A1 Antibody page for additional reagents targeting ANXA1 in inflammatory signaling, immune regulation and cancer biology.

Application Notes

Optimal dilution of the ANXA1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human Annexin A1 protein was used as the immunogen for the ANXA1 antibody.

Storage

Aliquot the ANXA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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