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Home >> Antibodies >> Anti-vWF Antibody

Anti-vWF Antibody [clone IIIE2.34] (V2929)

  Catalog No Formulation Size Price (USD)  
Image V2929-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2929-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2929SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-vWF antibody (clone IIIE2.34).
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-vWF antibody (clone IIIE2.34).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name IIIE2.34
Purity Protein G affinity chromatography
UniProt P04275
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-vWF antibody is available for research use only.
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Description

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Application Notes

Optimal dilution of the anti-vWF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunogen

A recombinant human protein fragment spanning aa 845-949 was used as the immunogen for the anti-vWF antibody.

Storage

Store the anti-vWF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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