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Home >> Antibodies >> Anti-Vimentin Antibody

Anti-Vimentin Antibody [clone VM1170] (V2928)

  Catalog No Formulation Size Price (USD)  
Image V2928-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2928-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2928SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2928IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human melanoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human uterus stained with anti-Vimentin antibody (clone VM1170).
Western blot testing of human HepG2 cell lysate with anti-Vimentin antibody. Predicted molecular weight ~58 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name VM1170
Purity Protein G affinity chromatography
UniProt P08670
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Vimentin antibody is available for research use only.
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Description

This mAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Application Notes

Optimal dilution of the anti-Vimentin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human protein was used as the immunogen for the anti-Vimentin antibody.

Storage

Store the anti-Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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