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Home >> Antibodies >> Anti-Vimentin Antibody

Anti-Vimentin Antibody (F48162)

  Catalog No Formulation Size Price (USD)  
F48162-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329

F48162-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Fluorescent confocal image of SY5Y cells stained with anti-Vimentin antibody at 1:100. Vimentin is localized to the cytoskeleton.
Western blot analysis of anti-Vimentin antibody and NCI-H460 lysate
Anti-Vimentin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue.
Anti-Vimentin antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken, Hamster, Mouse, Pig, Primate, Rat, Xenopus
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P08670
Applications Immunofluorescence : 1:100
Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This anti-Vimentin antibody is available for research use only.
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Description

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 430-457 from the human protein was used as the immunogen for this anti-Vimentin antibody.

Storage

Aliquot the anti-Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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