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Home >> Antibodies >> Anti-TGF-alpha Antibody

Anti-TGF-alpha Antibody [clone SPM542] (V9086)

  Catalog No Formulation Size Price (USD)  
Image V9086-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V9086-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V9086SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with anti-TGF alpha antibody (clone SPM542).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM, kappa
Clone Name SPM542
Purity Precipitated followed by dialysis in 1X PBS
UniProt P01135
Localization Cytoplasmic and Extracellular (secreted)
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-TGF-alpha antibody is available for research use only.
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Description

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Application Notes

The optimal dilution of the anti-TGF-alpha antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for this anti-TGF alpha antibody.

Storage

Store the anti-TGF-alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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