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Home >> Antibodies >> Anti-Tenascin C Antibody

Anti-Tenascin C Antibody [clone SPM319] (V2603)

  Catalog No Formulation Size Price (USD)  
Image V2603-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2603-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2603SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2603IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with anti-Tenascin C antibody (clone SPM319) at 4ug/ml.
SDS-PAGE analysis of purified, BSA-free anti-Tenascin C antibody (clone SPM319) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM319
Purity Protein G affinity chromatography
UniProt P24821
Localization Connective tissue matrix
Applications Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This anti-Tenascin C antibody is available for research use only.
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Description

Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Application Notes

Optimal dilution of the anti-Tenascin C antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human breast carcinoma cells were used as the immunogen for the anti-Tenascin C antibody.

Storage

Store the anti-Tenascin C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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