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Home >> Antibodies >> Anti-Prolactin Receptor Antibody

Anti-Prolactin Receptor Antibody [clone SPM213] (V9069)

  Catalog No Formulation Size Price (USD)  
Image V9069-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V9069-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V9069SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V9069IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with anti-Prolactin Receptor antibody (SPM213).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM213
Purity Protein G affinity chromatography
UniProt P16471
Localization Cell surface and cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Prolactin Receptor antibody is available for research use only.
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Description

It recognizes a protein of 70kDa, identified as prolactin receptor. Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.

Application Notes

The optimal dilution of the anti-Prolactin Receptor antibody for each application should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires digestion of tissue sections with Pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Semi-purified human prolactin receptor was used as the immunogen for this anti-Prolactin Receptor antibody.

Storage

Store the anti-Prolactin Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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