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Home >> Antibodies >> Anti-Perlecan Antibody (HSPG)

Anti-Perlecan Antibody (HSPG) [clone SPM255] (V2601)

  Catalog No Formulation Size Price (USD)  
Image V2601-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2601-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2601SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2601IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with anti-Perlecan antibody (clone SPM255).
IHC testing of FFPE human testicular carcinoma tissue with Perlecan antibody (clone SPM255).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (rat origin)
Isotype Rat IgG2a, kappa
Clone Name SPM255
Purity Protein G affinity chromatography
UniProt P98160
Localization Basement membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Perlecan antibody is available for research use only.
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Description

This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Application Notes

Optimal dilution of the anti-Perlecan antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A murine EHS laminin preparation was used as the immunogen for the anti-Perlecan antibody.

Storage

Store the anti-Perlecan antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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