• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Anti-PCNA Antibody

Anti-PCNA Antibody (F48250)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image F48250-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F48250-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
Bulk quote request
Western blot analysis of anti-PCNA antibody and 1) Jurkat, 2) HeLa, and 3) 293 lysate. Predicted molecular weight ~29kDa, routinely observed at 29~36kDa.
›
Western blot analysis of anti-PCNA antibody and 1) Jurkat, 2) HeLa, and 3) 293 lysate. Predicted molecular weight ~29kDa, routinely observed at 29~36kDa.
IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-PCNA antibody
Confocal immunofluorescent analysis of anti-PCNA antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Anti-PCNA antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken, Hamster, Mouse, Primate, Rat
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P12004
Applications Western Blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This anti-PCNA antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC-P
    Reactivity : Human
    Recrabbitmono
  • Applications : IHC-P, WB
    Reactivity : Human
    Recrabbitmono
  • Applications : FACS, IF, WB, IHC-P
    Reactivity : Human, Mouse, Rat
    Citation  Citations(11)
  • Applications : IHC, WB, ELISA
    Reactivity : Human, Mouse, Rat
    Pred. Reactivity : Bovine, Hamster, Primate
  • Applications : FACS, WB, IHC-P
    Reactivity : Human
  • Applications : IHC-P, WB
    Reactivity : Human
  • Applications : WB, IHC-P, FACS
    Reactivity : Human
  • Applications : IHC-P, WB
    Reactivity : Human, Mouse
    Rab Mono Image
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, FACS, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : IHC, IF, WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Bovine, Hamster, Primate, Rat
  • Applications : WB, ELISA (peptide), EIA
    Reactivity : Human, Mouse, Rat, Pig
    Pred. Reactivity : Dog, Cow
  • Applications : WB, ELISA (peptide), EIA
    Reactivity : Human, Mouse, Rat, Pig
    Pred. Reactivity : Dog, Cow
  • Applications : WB, ELISA (peptide), EIA
    Reactivity : Human, Mouse, Rat, Pig
    Pred. Reactivity : Dog, Cow
  • Applications : WB, IHC-P, FACS, IF, Direct ELISA
    Reactivity : Human, Mouse, Rat

Description

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

Application Notes

Titration of the anti-PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 89-117 from the human protein was used as the immunogen for this anti-PCNA antibody.

Storage

Aliquot the anti-PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all PCNA antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.