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Home >> Antibodies >> Anti-Nucleolin Antibody

Anti-Nucleolin Antibody [clone SPM614] (V2756)

  Catalog No Formulation Size Price (USD)  
Image V2756-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2756-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2756SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2756IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Immunofluorescent staining of PFA-fixed human HeLa cells with anti-Nucleolin antibody (clone SPM614, green) and DAPI (blue).
Immunofluorescent staining of PFA-fixed human K562 cells with anti-Nucleolin antibody (clone SPM614, green) and Phalloidin (red).
Immunofluorescent staining of PFA-fixed human MCF7 cells with anti-Nucleolin antibody (clone SPM614, green) and Phalloidin (red).
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-Nucleolin antibody (clone SPM614).
Flow cytometry testing of PFA fixed human K562 cells with anti-Nucleolin antibody (clone SPM614); Red=isotype control, Blue= anti-Nucleolin antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM614
Purity Protein G affinity chromatography
UniProt P19338
Localization Nucleoli
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-4ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Nucleolin antibody is available for research use only.
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Description

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Application Notes

Optimal dilution of the anti-Nucleolin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the anti-Nucleolin antibody.

Storage

Store the anti-Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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