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Home >> Antibodies >> Anti-NF-H Antibody

Anti-NF-H Antibody [clone SPM203] (V9060)

  Catalog No Formulation Size Price (USD)  
Image V9060-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V9060-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V9060SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V9060IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with anti-NF-H antibody (SPM203).
Western blot testing of human brain lysate with anti-NF-H antibody (clone SPM203).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM203
Purity Protein G affinity chromatography
UniProt P12036
Localization Cytoplasmic, membranous
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This anti-NF-H antibody is available for research use only.
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Description

This mAb reacts with a 200kDa protein, identified as heavy subunit of neurofilaments. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Application Notes

The optimal dilution of the anti-NF-H antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The Triton-X 100 insoluble protein fraction from rat brain was used as the immunogen for this anti-NF-H antibody.

Storage

Store the anti-NF-H antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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