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Home >> Antibodies >> Anti-Mitochondria Antibody

Anti-Mitochondria Antibody [clone SPM198] (V3079)

  Catalog No Formulation Size Price (USD)  
Image V3079-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3079-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3079SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V3079IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Formalin-fixed, paraffin-embedded human pancreas stained with anti-Mitochondria antibody (SPM198).
Immunofluorescent staining of fixed human HeLa cells with anti-Mitochondria antibody (clone SPM198, green) and Reddot nuclear stain.
Western blot testing of human HeLa cell lysate with anti-Mitochondria antibody (clone SPM198). Expected molecular weight ~60 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM198
Purity Protein G affinity chromatography
Localization Cytoplasmic (Mitochondria)
Applications Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Mitochondria antibody is available for research use only.
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Description

This mAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.

Application Notes

Optimal dilution of the anti-Mitochondria antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondria antibody.

Storage

Store the anti-Mitochondria antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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