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Home >> Antibodies >> Anti-L1 Antibody

Anti-L1 Antibody [clone SPM257] (V9117)

  Catalog No Formulation Size Price (USD)  
Image V9117-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V9117-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V9117SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC: Formalin-fixed, paraffin-embedded human cervix stained with anti-L1 antibody (SPM257).
Availability 1-3 business days
Species Reactivity Type 16 of human Papilloma Virus (HPV-16)
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name SPM257
Purity Protein G affinity chromatography
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-L1 antibody is available for research use only.
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Description

Reacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

Application Notes

The optimal dilution of the anti-L1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

Human papilloma virus type 16, major capsid protein L1 was used as the immunogen for this anti-L1 antibody.

Storage

Store the anti-L1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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