Anti-Insulin Antibody SPM531 / Mouse Monoclonal [clone SPM531] (V9035)

Description

Anti-Insulin Antibody SPM531 recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

For a highly published insulin monoclonal with an alternate INS05 clone designation, see our Insulin Antibody 2D11-H5 / INS05 Antibody page.

Application Notes

The optimal dilution of the Anti-Insulin Antibody SPM531 for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length (amino acids 1-84) purified pig insulin was used as the immunogen for this Anti-Insulin Antibody SPM531.

Storage

Store the Anti-Insulin Antibody SPM531 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).