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Home >> Antibodies >> Anti-HSP60 Antibody / HSPD1

Anti-HSP60 Antibody / HSPD1 [clone SPM253] (V2594)

  Catalog No Formulation Size Price (USD)  
Image V2594-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2594-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2594SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2594IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-HSP60 antibody (clone SPM253).
Formalin-fixed, paraffin-embedded human pancreas stained with anti-HSP60 antibody (clone SPM253).
Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP60 antibody (clone SPM253, green) and Reddot nuclear stain (red).
Western blot testing of human HeLa lysate with HSP60 antibody (clone SPM253). Predicted molecular weight: ~60 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM253
Purity Protein G affinity chromatography
UniProt P10809
Localization Cytoplasm (mitochondria)
Applications Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-HSP60 antibody is available for research use only.
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Description

Recognizes a 60kDa protein, identified as the heat shock protein 60 (Hsp60). Its epitope is localized between amino acids 383-447 of human Hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial Hsp60, but also against its mammalian homolog.

Application Notes

Optimal dilution of the anti-HSP60 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the anti-HSP60 antibody.

Storage

Store the anti-HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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