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Home >> Antibodies >> Anti-His Tag Antibody

Anti-His Tag Antibody (F52067)

  Catalog No Formulation Size Price (USD)  
Image F52067-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F52067-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of anti-His Tag antibody and a tagged recombinant protein.
Availability 1-3 business days
Species Reactivity NA
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Purified
Applications Western blot : 1:1000
Limitations This anti-His Tag antibody is available for research use only.
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Description

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin.

Application Notes

Titration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

HHHHHH peptide was used as the immunogen for the anti-His Tag antibody.

Storage

Aliquot the anti-His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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