- Tel: 858.663.9055
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- Tel: 858.663.9055
- Email: info@nsjbio.com
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Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin.
Titration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
HHHHHH peptide was used as the immunogen for the anti-His Tag antibody.
Aliquot the anti-His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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