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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Anti-His Antibody

Anti-His Antibody [clone 6AT18] (F52059)

  Catalog No Formulation Size Price (USD)  
Image F52059-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F52059-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of lysate from 12 tag recombinant protein using anti-His antibody at 1:1000.
Tagged recombinant protein tested with anti-His antibody.
Availability 1-3 business days
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 6AT18
Purity Purified
Applications Western blot : 1:1000-4000
Limitations This anti-His antibody is available for research use only.
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Description

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18, provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-His antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal anti-His antibody.

Storage

Store at 4oC for up to one month. For long-term, aliquot the anti-His antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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