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- Tel: 858.663.9055
- Email: info@nsjbio.com
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By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.
Store the anti-gp100 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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