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Home >> Antibodies >> Anti-Glycophorin A Antibody

Anti-Glycophorin A Antibody [clone SPM599] (V2563)

  Catalog No Formulation Size Price (USD)  
Image V2563-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2563-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2563SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2563IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human placenta stained with anti-Glycophorin A antibody (clone SPM599). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human angiosarcoma stained with anti-Glycophorin A antibody (clone SPM599). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM599
Purity Protein G affinity chromatography
UniProt P02724
Localization Cytoplasmic, membranous
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Glycophorin A antibody is available for research use only.
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Description

Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Application Notes

Optimal dilution of the anti-Glycophorin A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human GPA protein was used as the immunogen for the anti-Glycophorin A antibody.

Storage

Store the anti-Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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