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Home >> Antibodies >> Anti-GFP Antibody

Anti-GFP Antibody [clone 168AT1211.269.64] (F53773)

  Catalog No Formulation Size Price (USD)  
Image F53773-0.1ML In ascites with 0.09% sodium azide 0.1 ml 329
Western blot analysis of anti-GFP antibody using purified GFP/YFP/BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected.
Western blot analysis of anti-GFP antibody and recombinant protein.
Availability 1-3 business days
Species Reactivity GFP / YFP
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG
Clone Name 168AT1211.269.64
Purity Ascites
Applications Western blot : 1:100-1:500
Limitations This anti-GFP antibody is available for research use only.
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Description

Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection.

Application Notes

Titration of the anti-GFP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Purified His-tagged protein was used to produced this anti-GFP antibody.

Storage

Aliquot the anti-GFP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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