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Home >> Antibodies >> Anit-GAPDH Antibody

Anit-GAPDH Antibody (F50849)

  Catalog No Formulation Size Price (USD)  
Image F50849-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F50849-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of lysate from A375 cell line using anti-GAPDH antibody diluted at 1:500. Predicted molecular weight ~36kDa.
Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using anti-GAPDH antibody diluted at 1:1000 for each lane. Predicted molecular weight ~36kDa.
IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-GAPDH antibody
Western blot analysis of anti-GAPDH antibody and 1) A2058, 2) A375, and 3) CEM lysate. Predicted molecular weight ~36kDa.
Anti-GAPDH antibody confocal immunofluorescent analysis with HeLa cell. Primary antibody (1:20) was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of anti-GAPDH antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P04406
Applications Western blot : 1:500-1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This anti-GAPDH antibody is available for research use only.
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Description

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody.

Storage

Aliquot the anti-GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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