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Home >> Antibodies >> Anti-EpCAM Antibody

Anti-EpCAM Antibody [clone SPM134] (V2689)

  Catalog No Formulation Size Price (USD)  
Image V2689-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2689-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2689SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2689IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Immunofluorescent staining of human MCF7 cells with anti-EpCAM antibody (clone SPM134, green) and Reddot nuclear stain (red).
FACS surface testing of HT29 cells using PE conjugated anti-EpCAM antibody (red) and isotype control (green).
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with anti-EpCAM antibody (clone SPM134).
Flow cytometry testing of PFA-fixed human MCF7 cells with anti-EpCAM antibody (clone SPM134); Red=isotype control, Blue= anti-EpCAM antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM134
Purity Protein G affinity chromatography
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Flow cytometry : 1-2ug/million cells
Limitations This anti-EpCAM antibody is available for research use only.
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Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Application Notes

Optimal dilution of the anti-EpCAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Small cell lung carcinoma cells were used as the immunogen for the anti-EpCAM antibody.

Storage

Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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