EpCAM Antibody | Epithelial Cell Marker | EGP40/1372

	Catalog No	Formulation	Size	Price (USD)
	V3525-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V3525-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V3525SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation
	V3525IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	559
Formulation

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EpCAM Antibody Colorectal Carcinoma IF. Immunofluorescent staining of FFPE human colorectal carcinoma tissue using EpCAM Antibody / Epithelial Cell Marker Antibody demonstrates strong membranous green fluorescence outlining neoplastic epithelial cells throughout the tumor glands. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a cell surface glycoprotein involved in epithelial cell adhesion, tissue organization, and epithelial lineage maintenance. Prominent membrane-associated staining highlights the epithelial origin of the carcinoma cells and reflects the established use of EpCAM as a marker of epithelial differentiation and epithelial-derived malignancies. Nuclei are counterstained with Reddot (red), providing clear visualization of tissue architecture and cellular localization. The observed staining supports the utility of EpCAM for identification and characterization of epithelial tumor cell populations in colorectal carcinoma specimens.

EpCAM Antibody MCF-7 IF. Immunofluorescent staining of human MCF-7 breast carcinoma cells using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates strong green fluorescence outlining the cell membrane and cell-cell junctions of epithelial tumor cells. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein that mediates epithelial cell adhesion and contributes to maintenance of epithelial architecture. Prominent membrane localization highlights the cell surface distribution of EpCAM and is consistent with recognition of an extracellular domain epitope by clone EGP40/1372. Nuclei are counterstained with Reddot (red). The observed staining supports the use of EpCAM as a marker of epithelial lineage cells and epithelial-derived tumor cell populations in immunofluorescence applications.

EpCAM Antibody HEK293, A431 & HCT116 WB. Western blot analysis of human HEK293, A431, and HCT116 cell lysates using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates a strong immunoreactive band at approximately 35-40 kDa in all three cell lines. The observed molecular weight is consistent with Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein that commonly migrates above its calculated molecular mass due to N-linked glycosylation of the extracellular domain. EpCAM functions in epithelial cell adhesion, tissue organization, and cellular signaling, and is widely expressed by epithelial-derived cell lines and carcinomas. The robust signal observed across HEK293, A431, and HCT116 cells is consistent with established EpCAM expression in epithelial cell populations and supports the specificity of clone EGP40/1372 for detection of EPCAM by western blot. Expected molecular weight: ~35 kDa (unmodified), ~40-43 kDa (glycosylated).

EpCAM Antibody Hepatocellular Carcinoma IHC. Immunohistochemistry staining of FFPE human hepatocellular carcinoma tissue using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates strong membranous HRP-DAB brown staining throughout neoplastic epithelial cell populations. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a cell surface glycoprotein involved in epithelial cell adhesion, tissue organization, and intracellular signaling. Prominent circumferential membrane staining highlights the epithelial phenotype of the tumor cells and is characteristic of EpCAM expression in epithelial-derived malignancies. Because EpCAM is frequently associated with epithelial differentiation and tumor cell populations, its detection is widely utilized in cancer research and tumor characterization studies. Required HIER: steam tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris, 1 mM EDTA) for 10-20 minutes prior to staining.

EpCAM Antibody Colorectal Carcinoma IHC. Immunohistochemistry staining of FFPE human colorectal carcinoma tissue using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates strong membranous HRP-DAB brown staining throughout neoplastic gland-forming epithelial cells. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein involved in epithelial cell adhesion, maintenance of tissue architecture, and regulation of cellular signaling pathways. Prominent circumferential membrane staining highlights the epithelial origin of the carcinoma cells and reflects the established expression of EpCAM in colorectal epithelial tissues and colorectal carcinomas. The observed staining supports the use of EpCAM as a marker for epithelial differentiation, tumor cell identification, and characterization of epithelial-derived malignancies. Required HIER: steam tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris, 1 mM EDTA) for 10-20 minutes prior to staining.

EpCAM Antibody Human Lung WB. Western blot analysis of human lung tissue lysate using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates a distinct immunoreactive band at approximately 38-40 kDa. The observed molecular weight is consistent with Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein that frequently migrates above its predicted molecular mass due to N-linked glycosylation of the extracellular domain. EpCAM functions in epithelial cell adhesion, tissue organization, and regulation of cellular signaling pathways, and is expressed by epithelial cell populations in a variety of normal and neoplastic tissues. The detected band is consistent with the expected glycosylated form of EpCAM and supports the specificity of clone EGP40/1372 for western blot detection of EPCAM. Expected molecular weight: ~35 kDa (unmodified), ~40-43 kDa (glycosylated).

EpCAM Antibody FACS. Flow cytometry analysis using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates a pronounced rightward shift of the stained cell population (blue) relative to the negative control population (red), indicating specific cell surface binding to Epithelial Cell Adhesion Molecule (EPCAM/CD326). The observed fluorescence shift is consistent with expression of EpCAM, a transmembrane glycoprotein localized to the surface of epithelial cells where it functions in cell-cell adhesion, tissue organization, and epithelial signaling pathways. Recognition of an extracellular domain epitope by clone EGP40/1372 makes this antibody well suited for flow cytometric detection of viable epithelial cell populations and epithelial-derived tumor cells. The results support the utility of this antibody for identification, characterization, and isolation of EpCAM-positive cells in flow cytometry applications.

EpCAM Antibody Protein Microarray Validation. Analysis of a HuProt(TM) protein microarray containing more than 19,000 full-length human proteins using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates exceptional target specificity for Epithelial Cell Adhesion Molecule (EPCAM/CD326). EPCAM was identified as the top-ranked target with a Z-score of 127.85 and an S-score of 124.85, substantially exceeding all other proteins present on the array. The large separation between EPCAM and the next highest-ranking proteins indicates highly specific target recognition with minimal off-target binding. These results support the utility of clone EGP40/1372 for applications requiring high specificity, including immunohistochemistry, immunofluorescence, western blotting, and flow cytometry. The data demonstrate the foremost specificity of the EGP40/1372 monoclonal antibody for EPCAM.
Z- and S-score: The Z-score represents the strength of the signal generated when an antibody, in combination with a fluorescently labeled secondary antibody, binds to a specific protein on the HuProt(TM) array. Z-scores are expressed as standard deviations above the mean signal intensity of all proteins on the array. The S-score is the difference between the Z-scores of consecutive ranked targets and represents the relative specificity of the antibody for its intended target. An S-score of 2.5 or greater is generally considered indicative of target-specific antibody binding.

SDS-PAGE analysis of purified, BSA-free anti-EpCAM antibody (clone EGP40/1372) as confirmation of integrity and purity.

EpCAM Antibody Dog Bladder IHC. Immunohistochemistry staining of FFPE dog bladder tissue using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates strong membranous HRP-DAB brown staining throughout the urothelial epithelial lining. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein involved in epithelial cell adhesion, maintenance of tissue architecture, and regulation of epithelial signaling pathways. Prominent circumferential membrane staining highlights cell-cell junctions within the urothelium and is characteristic of EpCAM expression in epithelial tissues. The observed immunoreactivity supports the use of EpCAM as a marker of epithelial lineage cells and epithelial-derived tissues across species. HIER: steam tissue sections in pH 9 EDTA buffer for 10-20 minutes prior to staining.

EpCAM Antibody Cat Small Intestine IHC. Immunohistochemistry staining of FFPE cat small intestine tissue using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates strong membranous HRP-DAB brown staining throughout the intestinal epithelial lining, including absorptive enterocytes forming the glandular and villus-associated structures. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein that mediates epithelial cell adhesion and contributes to maintenance of epithelial architecture and tissue integrity. Prominent circumferential membrane staining highlights epithelial cell borders and is characteristic of EpCAM expression in gastrointestinal epithelial tissues. The observed immunoreactivity supports the use of EpCAM as a marker of epithelial lineage cells and epithelial-derived tissues across species. HIER: steam tissue sections in pH 9 EDTA buffer for 10-20 minutes prior to staining.

EpCAM Antibody MCF-7 WB. Western blot analysis of human MCF-7 breast carcinoma cell lysate using EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) demonstrates a prominent immunoreactive band at approximately 40-43 kDa. The observed molecular weight is consistent with the glycosylated form of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein expressed on epithelial cells and many epithelial-derived tumor cell lines. Additional weaker higher molecular weight signal may represent more extensively glycosylated EpCAM species, protein complexes, or processing intermediates commonly observed for membrane-associated glycoproteins. EpCAM functions in epithelial cell adhesion, maintenance of tissue architecture, and regulation of cellular signaling pathways. Detection in MCF-7 cells is consistent with the established expression of EpCAM in breast epithelial carcinoma models. Expected molecular weight: ~35 kDa (unmodified), ~40-43 kDa (glycosylated).

Availability	1-3 business days
Species Reactivity	Human, Dog, Cat
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	EGP40/1372
Purity	Protein G affinity chromatography
UniProt	P16422
Localization	Cell surface, cytoplasmic
Applications	Flow Cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This EpCAM Antibody / Epithelial Cell Marker Antibody is available for research use only.
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Description

EpCAM Antibody / Epithelial Cell Marker Antibody recognizes Epithelial Cell Adhesion Molecule (EPCAM), also known as EpCAM, CD326, and TACSTD1, a transmembrane glycoprotein expressed on the surface of many epithelial cells. EPCAM functions in cell-cell adhesion, tissue organization, epithelial integrity, and intracellular signaling, making it one of the most widely used markers for identification of epithelial lineage cells. Due to its abundant expression in epithelial tissues and many epithelial-derived tumors, EpCAM is extensively utilized in cancer research, stem cell biology, developmental biology, and studies of epithelial differentiation. Its extracellular localization further makes it particularly well suited for flow cytometry, cell sorting, and cell surface detection applications.

EpCAM is localized primarily to the plasma membrane, where it mediates homophilic cell-cell interactions and contributes to the maintenance of epithelial architecture. In addition to its adhesive properties, EPCAM participates in signaling pathways that regulate cellular proliferation, migration, differentiation, and survival. These functions allow EpCAM to influence tissue development and epithelial homeostasis beyond its structural role. Because expression is largely restricted to epithelial cell populations, EpCAM serves as a valuable marker for distinguishing epithelial cells from stromal, mesenchymal, and hematopoietic cell types in complex tissue environments.

EpCAM has become particularly important in cancer biology because it is frequently expressed by carcinomas arising from epithelial tissues. Researchers commonly use EPCAM antibodies to identify epithelial tumor cells, characterize tumor heterogeneity, evaluate epithelial differentiation status, and isolate circulating tumor cells from blood samples. The protein is widely employed in studies involving colorectal, breast, prostate, lung, pancreatic, ovarian, and other epithelial malignancies. As a result, EpCAM has become one of the most recognized epithelial markers in translational cancer research and tumor pathology investigations.

Beyond oncology applications, EPCAM plays important roles in embryonic development, tissue regeneration, epithelial repair, and stem cell biology. EpCAM expression is often associated with epithelial progenitor populations and actively proliferating epithelial compartments. Researchers therefore utilize EpCAM as a marker for epithelial lineage tracing, organoid development, regenerative medicine studies, and investigations of tissue homeostasis. The protein continues to serve as an important tool for understanding epithelial biology across a wide range of normal and pathological contexts.

At NSJ Bioreagents, we provide highly validated antibodies for cancer research, stem cell biology, developmental biology, and epithelial cell studies. EpCAM Antibody / Epithelial Cell Marker Antibody (clone EGP40/1372) recognizes an epitope within the extracellular domain of EPCAM and is validated for applications including IHC, IF, WB, flow cytometry, and protein microarray specificity analysis. This antibody is useful for investigating epithelial cell populations, epithelial-derived tumors, tissue organization, stem cell biology, and cell surface marker expression.

EpCAM regulates epithelial cell adhesion, tissue organization, and cell surface marker expression, making it an important target within our Cell Biology Antibodies collection.

Application Notes

Titering of the EpCAM Antibody / Epithelial Cell Marker Antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the EpCAM antibody.

Storage

Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

EPCAM Antibody, CD326 Antibody, Epithelial Cell Adhesion Molecule Antibody, TACSTD1 Antibody, Epithelial Marker Antibody, Cell Surface Glycoprotein Antibody

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EpCAM Antibody / Epithelial Cell Marker Antibody [clone EGP40/1372] (V3525)

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NSJ Bioreagents