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Home >> Antibodies >> Anti-EpCAM Antibody (Extracellular domain)

Anti-EpCAM Antibody (Extracellular domain) [clone EPM17-2] (V7241)

  Catalog No Formulation Size Price (USD)  
Image V7241-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V7241-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V7241SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC testing of human colorectal carcinoma with anti-EpCAM antibody (clone EPM17-2). Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human MCF-7 cells with EpCAM antibody (green, clone EPM17-2) and Reddot nuclear stain (red).
Western blot testing of human HCT116 cell lysate with EpCAM antibody (clone EPM17-2). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name EPM17-2
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Western blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1)
Limitations This anti-EpCAM antibody is available for research use only.
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Description

EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-EpCAM antibody to be titered up or down for optimal performance.

Immunogen

Human partial recombinant protein (extracellular portion) was used as the immunogen for this anti-EpCAM antibody.

Storage

Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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