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Home >> Antibodies >> Anti-EGFR Antibody

Anti-EGFR Antibody (F49183)

  Catalog No Formulation Size Price (USD)  
Image F49183-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F49183-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of lysate from A431, HUVEC, PC3 cell line (left to right) using anti-EGFR antibody diluted at 1:1000 for each lane.
Anti-EGFR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue.
Anti-EGFR antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P00533
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This anti-EGFR antibody is available for research use only.
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Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Application Notes

Titration of the anti-EGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1004-1033 from the human protein was used as the immunogen for this anti-EGFR antibody.

Storage

Aliquot the anti-EGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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