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Home >> Antibodies >> Anti-E Cadherin Antibody

Anti-E Cadherin Antibody (F44157)

  Catalog No Formulation Size Price (USD)  
Image F44157-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F44157-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of anti-E Cadherin antibody and A375 lysate. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Western blot analysis of anti-E Cadherin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (2). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Anti-E Cadherin antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P12830
Localization Cytoplasmic, membranous
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This anti-E Cadherin antibody is available for research use only.
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Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. E Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. [UniProt]

Application Notes

Titration of the anti-E Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 833-862 from the human protein was used as the immunogen for this anti-E Cadherin antibody.

Storage

Aliquot the anti-E Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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