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Home >> Antibodies >> Anti-Cytokeratin 18 Antibody

Anti-Cytokeratin 18 Antibody [clone SPM510] (V9041)

  Catalog No Formulation Size Price (USD)  
Image V9041-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V9041-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V9041SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V9041IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Flow cytometry testing of PFA-fixed human HeLa cells with anti-Cytokeratin 18 antibody (clone SPM510); Red=isotype control, Blue= anti-Cytokeratin 18 antibody.
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with anti-Cytokeratin 18 antibody (clone SPM510).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM510
Purity Protein G affinity chromatography
UniProt P05783
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Cytokeratin 18 antibody is available for research use only.
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Description

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Application Notes

The optimal dilution of the anti-Cytokeratin 18 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human breast cancer PMC 42 cells were used as the immunogen for this anti-Cytokeratin 18 antibody.

Storage

Store the anti-Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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