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Home >> Antibodies >> Anti-CD79a Antibody

Anti-CD79a Antibody [clone SPM550] (V9110)

  Catalog No Formulation Size Price (USD)  
Image V9110-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V9110-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V9110SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V9110IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Immunofluorescent staining of PFA-fixed human Raji cells with CD79 antibody (clone SPM550, green) and Reddot nuclear stain (red).
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD79a antibody (clone SPM550).
Western blot testing of human Raji cell lysate with CD79 antibody (clone SPM550). Expected molecular weight: 25-47 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM550
Purity Protein G affinity chromatography
UniProt P11912
Localization Cell surface, cytoplasmic
Applications Western blot : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-CD79a antibody is available for research use only.
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Description

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

Application Notes

The optimal dilution of the anti-CD79a antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 202-216 (GTYQDVGSLNIADVQ) of the human protein was used as the immunogen for this anti-CD79a antibody.

Storage

Store the anti-CD79a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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