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Home >> Antibodies >> Anti-CD57 Antibody

Anti-CD57 Antibody [clone SPM527] (V2531)

  Catalog No Formulation Size Price (USD)  
Image V2531-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2531-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2531SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2531IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD57 antibody (SPM527).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM, kappa
Clone Name SPM527
Purity PEG precipitation
UniProt Q9P2W7
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This anti-CD57 antibody is available for research use only.
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Description

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Application Notes

Optimal dilution of the anti-CD57 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human peripheral blood mononuclear cells were used as the immunogen for the anti-CD57 antibody.

Storage

Store the anti-CD57 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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