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Home >> Antibodies >> Anti-CD45RO Antibody

Anti-CD45RO Antibody [clone SPM125] (V2420)

  Catalog No Formulation Size Price (USD)  
Image V2420-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2420-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2420SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2420IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of anti-CD45RO antibody and FFPE human lymphoma
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name SPM125
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P08575
Localization Cell surface
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-CD45RO antibody is available for research use only.
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Description

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Application Notes

Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.

Storage

The anti-CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

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