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Home >> Antibodies >> Anti-CD33 Antibody

Anti-CD33 Antibody (R32381)

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  Catalog No Formulation Size Price (USD)  
Image R32381 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human SKOV3 cell lysate with anti-CD33 antibody. Predicted molecular weight is 40-67 kDa depending on glycosylation level.
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Western blot testing of human SKOV3 cell lysate with anti-CD33 antibody. Predicted molecular weight is 40-67 kDa depending on glycosylation level.
IHC testing of FFPE human tonsil with anti-CD33 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Flow cytometry testing of human U937 cells with CD33 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD33 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P20138
Localization Cytoplasmic, membranous
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This anti-CD33 antibody is available for research use only.
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Description

CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.

Application Notes

Optimal dilution of the anti-CD33 antibody should be determined by the researcher.

Immunogen

Amino acids D18-H259 of the human protein were used as the immunogen for the anti-CD33 antibody.

Storage

After reconstitution, the anti-CD33 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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