Calponin Antibody Mouse Monoclonal [clone CNN1/832] (V2465)

Description

Calponin antibody recognizes Calponin 1, an actin-binding protein encoded by the CNN1 gene and a widely used marker of smooth muscle and myoepithelial differentiation. Calponin Antibody Mouse Monoclonal (clone CNN1/832) is developed for research applications evaluating contractile smooth muscle cells in normal and neoplastic tissues. Calponin 1 localizes predominantly to the cytoplasm of smooth muscle cells, where it associates with actin filaments and regulates contractile activity and cytoskeletal organization.

Calponin antibody, also referred to as Calponin 1 antibody, CNN1 antibody, and basic calponin antibody in the literature, targets a member of the calponin family of actin-binding proteins. Calponin 1 contains calponin homology domains and actin-binding regions that facilitate interaction with actin, tropomyosin, and other contractile proteins. Through these interactions, Calponin 1 modulates actomyosin ATPase activity and supports maintenance of the differentiated contractile phenotype in smooth muscle cells.

CNN1 expression is characteristic of vascular smooth muscle, gastrointestinal smooth muscle, uterine myometrium, and myoepithelial cells in glandular tissues such as breast and salivary gland. The expected immunostaining pattern is cytoplasmic positivity in smooth muscle layers and myoepithelial cell populations. Because of this lineage-restricted distribution, Calponin 1 serves as a reliable research marker for identifying smooth muscle and myoepithelial differentiation in tissue-based studies.

In tumor research, Calponin 1 expression is frequently evaluated in leiomyoma, leiomyosarcoma, myoepithelial tumors, and other mesenchymal neoplasms. Cytoplasmic staining supports smooth muscle or myoepithelial lineage, whereas most epithelial carcinomas and non-mesenchymal malignancies demonstrate limited or absent expression. Calponin Antibody Mouse Monoclonal (clone CNN1/832) enables consistent detection of Calponin 1 expression patterns in research settings at NSJ Bioreagents.

Application Notes

Optimal dilution of the anti-Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the anti-Calponin antibody.

Storage

Store the anti-Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).