Calponin 1 Antibody / Smooth Muscle Contractile Marker [clone CNN1/832] (V2465)

Description

Calponin 1 (CNN1), also known as Calponin, is an actin-binding protein that plays a central role in smooth muscle contraction and cytoskeletal organization. The Calponin 1 Antibody / Smooth Muscle Contractile Marker is designed to detect CNN1 in pathways associated with smooth muscle differentiation, contractile function, and cytoskeletal regulation across multiple experimental systems. CNN1 is a well-established marker of differentiated smooth muscle cells, where it contributes to maintenance of contractile phenotype and structural integrity.

Calponin 1 antibody, also referred to as CNN1 antibody or Calponin antibody in the literature, enables detection of this protein across a wide range of assay platforms, including immunohistochemistry, western blot, immunofluorescence, and flow cytometry. CNN1 expression is highly enriched in smooth muscle tissues such as vascular smooth muscle, gastrointestinal muscularis layers, and other contractile structures, making it a reliable marker for identifying smooth muscle lineage in both normal and pathological contexts. Its expression pattern is frequently used to distinguish smooth muscle cells from surrounding stromal, epithelial, or fibroblast populations.

Functionally, Calponin 1 binds to actin filaments and regulates actomyosin ATPase activity, thereby modulating contractile force generation. In addition to its role in contraction, CNN1 contributes to cytoskeletal stability and organization, linking contractile signaling pathways to structural support within the cell. These functions make Calponin 1 Antibody valuable for studies of muscle physiology, vascular biology, and smooth muscle-associated disease processes, including conditions involving altered contractility or cellular differentiation.

Subcellularly, CNN1 localizes along actin filaments within the cytoplasm, producing a filament-associated distribution that can be visualized in immunofluorescence and a diffuse cytoplasmic staining pattern in immunohistochemistry. In western blot analysis, detection of CNN1 confirms protein expression in relevant tissues and cell lines, while flow cytometry applications, when combined with appropriate intracellular staining protocols, allow identification of CNN1-positive cell populations. These complementary approaches enable both structural and quantitative assessment of protein expression.

This Calponin 1 antibody is supported by immunohistochemistry, western blot, and immunofluorescence data, along with flow cytometry analysis performed using the recombinant form of clone CNN1/832. These combined validation approaches demonstrate reliable detection across multiple assay formats and biological contexts, supporting its use in studies of smooth muscle biology, cytoskeletal organization, and contractile function.

This Calponin 1 antibody is part of a broader antibody panel offered by NSJ Bioreagents.

Application Notes

Optimal dilution of the Calponin 1 Antibody / Smooth Muscle Contractile Marker should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the Calponin 1 antibody.

Storage

Store the Calponin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

CNN1 antibody, Calponin antibody, Calponin 1 protein antibody, Smooth muscle calponin antibody, CNN1 protein antibody