• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Anti-Biotin Antibody

Anti-Biotin Antibody [clone SPM375] (V9113)

  Catalog No Formulation Size Price (USD)  
Image V9113-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
Image
V9113-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
Image
V9113SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375).
Availability 1-3 business days
Species Reactivity Biotin
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM375
Purity Protein G affinity chromatography
Localization target location
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Biotin antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Application Notes

The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Immunogen

Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.

Storage

Store the anti-Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.