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Home >> Antibodies >> Anti-Bcl-2 Antibody

Anti-Bcl-2 Antibody [clone SPM530] (V9074)

  Catalog No Formulation Size Price (USD)  
Image V9074-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V9074-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V9074SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V9074IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC analysis of formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with anti-Bcl-2 antibody (clone SPM530).
Western blot of testing of human skin lysate (left) and human MCF7 cell lysate (right) using anti-Bcl-2 antibody (clone SPM530).
FACS staining (intracellular) of Jurkat cells using anit-Bcl-2 antibody (red) and isotype control (green).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM530
Purity Protein G affinity chromatography
UniProt P10415
Localization Cytoplasmic, membrane
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-Bcl-2 antibody is available for research use only.
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Description

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Application Notes

The optimal dilution of the anti-Bcl-2 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for this anti-Bcl-2 antibody.

Storage

Store the anti-Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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