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Home >> Antibodies >> Anti-ATG5 Antibody

Anti-ATG5 Antibody (F46219)

  Catalog No Formulation Size Price (USD)  
Image F46219-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F46219-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Fluorescent image of U251 cells stained with anti-ATG5 antibody diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
Western blot analysis of anti-ATG5 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
IHC analysis of FFPE human hepatocarcinoma tissue stained with the anti-ATG5 antibody
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q9H1Y0
Applications Immunofluorescence : 1:25
Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This anti-ATG5 antibody is available for research use only.
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Description

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Application Notes

Titration of the anti-ATG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 209-238 from the human protein was used as the immunogen for this anti-ATG5 antibody.

Storage

Aliquot the anti-ATG5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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