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Home >> Antibodies >> Anti-Alkaline Phosphatase Antibody (intestinal/placental)

Anti-Alkaline Phosphatase Antibody (intestinal/placental) (F44184)

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  Catalog No Formulation Size Price (USD)  
Image F44184-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F44184-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from human placenta tissue lysate using anti-Alkaline Phosphatase antibody diluted at 1:1000.
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Western blot analysis of lysate from human placenta tissue lysate using anti-Alkaline Phosphatase antibody diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded human small intestine section using anti-Alkaline Phosphatase antibody diluted at 1:100 dilution.
Fluorescent confocal image of HeLa cells stained with anti-Alkaline Phosphatase antibody at 1:100. Immunoreactivity is localized to the cytoplasm.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P09923
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-100
Immunofluorescence : 1:100
Limitations This anti-Alkaline Phosphatase antibody is available for research use only.
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Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.

Application Notes

Titration of the anti-Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 274-304 from human ALPI was used as the immunogen for this anti-Alkaline Phosphatase antibody. This sequence is 87% homologous in human ALPP.

Storage

Aliquot the anti-Alkaline Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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