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Home >> Antibodies >> Anti-Actin Antibody / ACTA1

Anti-Actin Antibody / ACTA1 (R32166)

  Catalog No Formulation Size Price (USD)  
Image R32166 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 359
Western blot testing of 1) rat liver, 2) rat kidney, 3) mouse spleen, human 4) HeLa, 5) COLO320, 6) SMMC and 7) A549 lysate with anti-Actin antibody. Expected molecular weight: 42-45 kDa.
IHC testing of FFPE human breast cancer tissue with anti-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse intestine with anti-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat speen with anti-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P68133
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This anti-Actin antibody is available for research use only.
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Description

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Application Notes

Optimal dilution of the anti-Actin antibody should be determined by the researcher.

Immunogen

Amino acids ITKQEYDEAGPSIVHRKCF of human ACTA1 were used as the immunogen for the anti-Actin antibody.

Storage

After reconstitution, the anti-Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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