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Home >> Antibodies >> Anti-Actin Antibody / ACTA1

Anti-Actin Antibody / ACTA1 (R32166)

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Image R32166 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat heart, 2) rat brain, 3) rat kidney, 4) mouse heart, 5) mouse brain, 6) mouse spleen and 7) mouse kidney tissue lysate with anti-Actin antibody. Expected molecular weight: 42-45 kDa.
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Western blot testing of 1) rat heart, 2) rat brain, 3) rat kidney, 4) mouse heart, 5) mouse brain, 6) mouse spleen and 7) mouse kidney tissue lysate with anti-Actin antibody. Expected molecular weight: 42-45 kDa.
Western blot testing of human 1) placenta, 2) HeLa, 3) HepG2, 4) 293T, 5) A431, 6) U-87 MG, 7) U937 and 8) K562 cell lysate with anti-Actin antibody. Expected molecular weight: 42-45 kDa.
IHC staining of FFPE human placental tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P68133
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This anti-Actin antibody is available for research use only.
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Description

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Application Notes

Optimal dilution of the anti-Actin antibody should be determined by the researcher.

Immunogen

Amino acids ITKQEYDEAGPSIVHRKCF of human ACTA1 were used as the immunogen for the anti-Actin antibody.

Storage

After reconstitution, the anti-Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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