ANP Antibody / ANF / Atrial Natriuretic Peptide (RQ4454)

Western blot testing of two lots of rat heart lysate (lanes 1 & 2) and two lots of mouse heart lysate (lanes 3 & 4) with ANP antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.

ANP Antibody Mouse Heart IHC. Immunohistochemistry staining of FFPE mouse heart tissue using ANP antibody demonstrates localized cytoplasmic staining within cardiac-associated cell populations, consistent with expression of Atrial Natriuretic Peptide (ANP/ANF). ANP is a peptide hormone encoded by the NPPA gene and functions as a key regulator of cardiovascular homeostasis, fluid balance, and natriuretic peptide signaling. The observed staining pattern is consistent with the established role of cardiac tissue as a primary site of ANP production and endocrine activity. These findings support the utility of ANP antibody for studies of cardiac hormone biology, cardiovascular signaling pathways, and endocrine regulation. HIER: boil tissue sections in pH6 10mM citrate buffer for 10-20 min followed by cooling at room temperature for 20 min.

ANP Antibody Rat Heart IHC. Immunohistochemistry staining of FFPE rat heart tissue using ANP antibody demonstrates localized cytoplasmic staining within cardiac muscle-associated cell populations, consistent with expression of Atrial Natriuretic Peptide (ANP/ANF). ANP is a cardiac-derived peptide hormone encoded by the NPPA gene that participates in natriuretic peptide signaling pathways regulating cardiovascular homeostasis, circulatory balance, and endocrine communication. The observed staining pattern is consistent with the established role of cardiac tissue as a major source of ANP production and secretion. These findings support the utility of ANP antibody for studies of cardiac endocrine function, cardiovascular signaling, and peptide hormone biology. HIER: boil tissue sections in pH6 10mM citrate buffer for 10-20 min followed by cooling at room temperature for 20 min.

ANP Antibody Rat Small Intestine IHC. Immunohistochemistry staining of FFPE rat small intestine tissue using ANP antibody demonstrates localized staining within submucosal and stromal-associated cellular structures, consistent with expression of Atrial Natriuretic Peptide (ANP/ANF). ANP is a peptide hormone encoded by the NPPA gene that functions as an important regulator of cardiovascular homeostasis, fluid balance, and endocrine signaling. While ANP is most prominently associated with cardiac tissue, natriuretic peptide signaling components are also present in extra-cardiac tissues where they may participate in local regulatory and homeostatic processes. These findings support the utility of ANP antibody for studies of peptide hormone expression, endocrine communication, and natriuretic peptide biology. HIER: boil tissue sections in pH6 10mM citrate buffer for 10-20 min followed by cooling at room temperature for 20 min.