Annexin VI Antibody / ANXA6 Polyclonal Antibody (RQ4425)

Annexin VI Antibody IHC Staining of Human Lung Cancer Tissue. Immunohistochemical analysis of FFPE human lung cancer tissue using ANXA6 Polyclonal Antibody. Brown DAB staining is visible along cellular boundaries and within portions of the cytoplasm, particularly among cells surrounding the alveolar spaces. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody IHC Staining of Human Thyroid Cancer Tissue. Immunohistochemical analysis of FFPE human thyroid cancer tissue using ANXA6 Polyclonal Antibody. Brown DAB staining is present in the epithelial cells lining glandular structures, with cytoplasmic and peripheral cellular staining visible against the blue hematoxylin counterstain. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody IHC Staining of Rat Colon Tissue. Immunohistochemical analysis of FFPE rat colon tissue using ANXA6 Polyclonal Antibody. Brown DAB staining is distributed among epithelial cells of the colonic glands, with additional staining visible in portions of the surrounding tissue. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody IHC Staining of Mouse Lymph Tissue. Immunohistochemical analysis of FFPE mouse lymph tissue using ANXA6 Polyclonal Antibody. Brown DAB staining is visible in scattered cells and cellular regions throughout the lymphoid tissue, against a predominantly blue hematoxylin counterstain. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody Western Blot Analysis of Human, Rat and Mouse Lysates. Western blot analysis using ANXA6 Polyclonal Antibody at 0.5 ug/ml. Lane 1: human placenta; lane 2: human Jurkat cells; lane 3: human HeLa cells; lane 4: human HepG2 cells; lane 5: rat liver; lane 6: rat lung; lane 7: mouse liver; lane 8: mouse lung. Immunoreactive bands are detected in the approximately 67-76 kDa region, consistent with the expected molecular weight range of annexin A6.

Annexin VI Antibody Flow Cytometry Analysis of Human HL60 Cells. Flow cytometric analysis of fixed and permeabilized human HL60 cells using ANXA6 Polyclonal Antibody at 1 ug per 10^6 cells following blocking with goat serum. The red histogram represents cells alone, the green histogram represents the isotype control, and the blue histogram represents antibody-stained cells. The rightward shift of the blue population relative to both controls indicates detectable intracellular ANXA6 immunoreactivity.

Annexin VI Antibody Western Blot Validation in HEK293 Wild-Type and ANXA6 Knockout Cells. Western blot analysis of human HEK293 wild-type (WT) and ANXA6 knockout (KO) whole-cell lysates using ANXA6 Polyclonal Antibody at 0.5 ug/ml. Lane 1 contains WT lysate and lane 2 contains ANXA6 KO lysate, with 30 ug of total protein loaded per lane under reducing conditions. An immunoreactive band at approximately 68-76 kDa is detected in the WT sample and is absent or markedly reduced in the KO sample, supporting antibody specificity for ANXA6. GAPDH serves as the loading control.