Annexin VI Antibody 4C4 / ANXA6 Monoclonal Antibody [clone 4C4] (RQ6590)

Annexin VI Antibody 4C4 IHC Staining of Human Renal Clear Cell Carcinoma. Immunohistochemical analysis of FFPE human renal clear cell carcinoma tissue using ANXA6 Monoclonal Antibody clone 4C4. Brown DAB staining is visible in scattered tumor cells, with cytoplasmic and peripheral cellular staining against a blue hematoxylin counterstain. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody 4C4 IHC Staining of Human Lung Cancer Tissue. Immunohistochemical analysis of FFPE human lung cancer tissue using ANXA6 Monoclonal Antibody clone 4C4. Brown DAB staining is visible along portions of the cellular boundaries and within scattered cells, with variable staining across the tissue section. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody 4C4 IHC Staining of Human Liver Tissue. Immunohistochemical analysis of FFPE human liver tissue using ANXA6 Monoclonal Antibody clone 4C4. Brown DAB staining is visible in hepatocytes, particularly along cellular boundaries and within portions of the cytoplasm. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody 4C4 IHC Staining of Mouse Pancreas Tissue. Immunohistochemical analysis of FFPE mouse pancreas tissue using ANXA6 Monoclonal Antibody clone 4C4. Brown DAB staining is observed in pancreatic acinar cells, with prominent peripheral and cytoplasmic immunoreactivity surrounding the glandular lumens. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody 4C4 IHC Staining of Rat Pancreas Tissue. Immunohistochemical analysis of FFPE rat pancreas tissue using ANXA6 Monoclonal Antibody clone 4C4. Brown DAB staining is distributed throughout the pancreatic acinar tissue, with cytoplasmic and peripheral cellular immunoreactivity visible in numerous glandular cells. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes, followed by cooling before staining.

Annexin VI Antibody 4C4 IF Staining of Human T-47D Cells. Immunofluorescence analysis of FFPE human T-47D cells using ANXA6 Monoclonal Antibody clone 4C4. Green fluorescence indicates ANXA6 immunoreactivity distributed predominantly throughout the cytoplasm, while DAPI counterstaining identifies cell nuclei in blue. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes.

Annexin VI Antibody 4C4 Western Blot Analysis of Human, Rat and Mouse Lysates. Western blot analysis using ANXA6 Monoclonal Antibody clone 4C4. Lane 1: human placenta; lane 2: human U-87 MG cells; lane 3: human HepG2 cells; lane 4: human HeLa cells; lane 5: rat thymus; lane 6: rat pancreas; lane 7: mouse thymus; lane 8: mouse pancreas. Immunoreactive bands are detected near 70 kDa across all eight samples, consistent with the expected molecular weight range of annexin A6 (67-76 kDa).

Annexin VI Antibody 4C4 Flow Cytometry Analysis of Human U-87 MG Cells. Flow cytometric analysis of human U-87 MG cells using ANXA6 Monoclonal Antibody clone 4C4 at 1 ug per million cells following blocking with goat serum. The red histogram represents cells alone, the green histogram represents the isotype control, and the blue histogram represents antibody-stained cells. The rightward shift of the blue histogram relative to both controls demonstrates detectable ANXA6-associated fluorescence.