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Home >> Antibodies >> Annexin V Antibody

Annexin V Antibody (R31475)

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  Catalog No Formulation Size Price (USD)  
Image R31475 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of Annexin V antibody and rat samples 1: lung; 2: brain; 3: heart; 4: kidney; and human samples 5: HeLa; 6: SMMC-7721; 7: A549; 8: SGC lysate. Predicted molecular weight ~36 kDa.
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Western blot testing of Annexin V antibody and rat samples 1: lung; 2: brain; 3: heart; 4: kidney; and human samples 5: HeLa; 6: SMMC-7721; 7: A549; 8: SGC lysate. Predicted molecular weight ~36 kDa.
IHC-P: Annexin V antibody testing of human breast cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC-P: Annexin V antibody testing of human intestinal cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC-P: Annexin V antibody testing of mouse kidney tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC-P: Annexin V antibody testing of rat kidney tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of frozen human placenta with Annexin V antibody.
IHC staining of frozen rat kidney with Annexin V antibody.
Immunofluorescent staining of FFPE human placenta with Annexin V antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with Annexin V antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat RH35 cells with Annexin V antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human PC-3 cells with Annexin V antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin V antibody.
Flow cytometry testing of human SiHa cells with Annexin V antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin V antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 308
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Annexin V antibody is available for research use only.
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Description

Annexin V or A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an Annexin V shield blocks the entry of phospholipids into coagulation (clotting) reactions. Annexin AV can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Annexin V antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 2-320) was used as the immunogen for this Annexin V antibody.

Storage

After reconstitution, the Annexin V antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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