Annexin A3 Antibody / Calcium Binding Protein Antibody (R31948)

Annexin A3 Antibody Human Gastric Carcinoma IHC. Immunohistochemical staining of FFPE human gastric carcinoma tissue using Annexin A3 antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer before incubation with Annexin A3 antibody at 2 ug/ml. An HRP-conjugated secondary antibody with DAB chromogen was used for visualization. Tumor cells exhibit strong cytoplasmic and membranous staining, consistent with the membrane-associated functions of Annexin A3 in calcium-dependent phospholipid binding and vesicle trafficking. This Annexin A3 Antibody, also called Calcium Binding Protein Antibody, is suitable for studies of calcium-dependent phospholipid binding, membrane trafficking, vesicle dynamics, and cancer biology.

Annexin A3 Antibody HeLa Cell IF. Immunofluorescent staining of HeLa cells using Annexin A3 antibody. Cells were treated with enzymatic antigen retrieval for 15 minutes before incubation with Annexin A3 antibody at 5 ug/ml. A Fluoro488-conjugated secondary antibody was used to detect Annexin A3 (green), and nuclei were counterstained with DAPI (blue). Annexin A3 staining is predominantly cytoplasmic with enrichment along the cell periphery, consistent with its role as a calcium-dependent phospholipid-binding protein involved in membrane trafficking and membrane dynamics. This Annexin A3 Antibody, also called Calcium Binding Protein Antibody, is suitable for studies of calcium-dependent phospholipid binding, membrane trafficking, vesicle dynamics, and cancer biology.

Annexin A3 Antibody Human, Rat and Mouse WB. Western blot analysis of Annexin A3 expression in human cell lines and rodent tissues using Annexin A3 antibody. Lane 1: A431; Lane 2: Caco-2; Lane 3: SiHa; Lane 4: HeLa; Lane 5: rat lung; Lane 6: rat kidney; Lane 7: mouse lung; Lane 8: mouse kidney. Proteins were resolved on a 10% SDS-PAGE gel, transferred to nitrocellulose, and the membrane was incubated with Annexin A3 antibody at 0.5 ug/ml followed by an HRP-conjugated secondary antibody. A specific band was detected at the expected molecular weight of approximately 36 kDa in all samples examined. This Annexin A3 Antibody, also called Calcium Binding Protein Antibody, is suitable for studies of calcium-dependent phospholipid binding, membrane trafficking, vesicle dynamics, and cancer biology.

Annexin A3 Antibody HeLa Cell FACS. Flow cytometric analysis of fixed and permeabilized HeLa cells using Annexin A3 antibody. Cells were incubated with Annexin A3 antibody at 1 ug/10^6 cells followed by a Fluoro488-conjugated secondary antibody. The blue histogram (Annexin A3 antibody) shows a clear rightward shift relative to the rabbit IgG isotype control (green) and the unstained control (red), demonstrating specific detection of endogenous Annexin A3 expression. This Annexin A3 Antibody, also called Calcium Binding Protein Antibody, is suitable for studies of calcium-dependent phospholipid binding, membrane trafficking, vesicle dynamics, and cancer biology.

Annexin A3 Antibody A431 Cell IP. Immunoprecipitation of Annexin A3 from A431 whole cell lysate using Annexin A3 antibody. Input: A431 whole cell lysate (30 ug). IP Control: rabbit IgG with A431 lysate. IP: 2 ug Annexin A3 antibody incubated with 500 ug A431 whole cell lysate. Immunoprecipitated proteins were analyzed by Western blot using the same Annexin A3 antibody at 0.5 ug/ml. A specific band corresponding to Annexin A3 was detected in the immunoprecipitated sample, demonstrating successful target enrichment, while the IgG heavy chain is visible at approximately 50 kDa. This Annexin A3 Antibody, also called Calcium Binding Protein Antibody, is suitable for studies of calcium-dependent phospholipid binding, membrane trafficking, vesicle dynamics, and cancer biology.