Annexin A1 Antibody (R31548)

Annexin A1 Antibody Human Tonsil IHC. Immunohistochemistry staining of FFPE human tonsil tissue was performed with Annexin A1 Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8, for 20 min followed by cooling. Positive staining is observed in populations of immune cells and surrounding tissue compartments. This Annexin A1 Antibody demonstrates ANXA1 expression in human tonsil and supports studies of Annexin A1 in immune and inflammatory signaling.

Annexin A1 Antibody Rat Lung Bronchial Epithelium IHC. Immunohistochemistry staining of FFPE rat lung tissue was performed with Annexin A1 Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8, for 20 min followed by cooling. Strong positive staining is observed in the bronchial epithelial cells lining the airway. This Annexin A1 Antibody demonstrates ANXA1 expression in rat lung and supports studies of Annexin A1 in pulmonary and inflammatory biology.

Annexin A1 Antibody Human, Monkey and Rat Sample WB. Western blot analysis was performed with Annexin A1 Antibody using 1) human HeLa, 2) human K562, 3) monkey COS-7, 4) human A549, 5) human PC-3, 6) human U-2 OS, 7) rat spleen, 8) rat lung, 9) rat kidney and 10) rat C6 lysates. A prominent band is detected near the predicted molecular weight of approximately 38 kDa across the tested samples. This Annexin A1 Antibody demonstrates ANXA1 detection across multiple human, monkey and rat cell and tissue lysates.

Annexin A1 Antibody Human, Monkey, Rat and Mouse WB. Western blot analysis of Annexin A1 was performed using human U87, HeLa and K562 cells, monkey COS7 cells, rat lung and spleen, and mouse lung lysates. Lane 1: U87; Lane 2: HeLa; Lane 3: K562; Lane 4: COS7; Lane 5: rat lung; Lane 6: rat spleen; Lane 7: mouse lung. Samples were loaded at 30 ug per lane, and the rabbit polyclonal Annexin A1 Antibody was used at 0.5 ug/ml overnight at 4oC. A prominent Annexin A1 band is detected at approximately 36 kDa, near the predicted molecular weight of approximately 39 kDa, demonstrating ANXA1 detection across human, monkey, rat and mouse samples.

Annexin A1 Antibody Human and Monkey Cell WB. Western blot analysis of Annexin A1 was performed using human U-87 MG, HeLa and K562 cell lysates and monkey COS-7 cell lysate. Lane 1: U-87 MG; Lane 2: HeLa; Lane 3: K562; Lane 4: COS-7. Samples were loaded at 30 ug per lane, and the rabbit polyclonal Annexin A1 Antibody was used at 0.5 ug/ml overnight at 4oC, followed by DyLight 647-conjugated secondary antibody detection. A prominent band is detected at approximately 35 kDa in all four cell lysates, near the predicted molecular weight of approximately 39 kDa, demonstrating ANXA1 detection in human and monkey cells.